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Year : 2010  |  Volume : 8  |  Issue : 15  |  Page : 14-23

Microbial contamination of dental unit water lines: Evaluation, disinfection and recurrence

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Background: Several studies have shown presence of microorganisms in Dental Unit Water Lines (DUWLs) above the recommended standard of American Dental Association (ADA). Objectives: (i) To evaluate the microbial contamination of DUWLs of departments of College of Dental Sciences, Mobile Dental Van (MDV) and Private dental clinics of Davangere city. (ii) To compare efficacy of Sterilex Ultra & 0.2% Chlorhexidine Gluconate (CHX) mouthwash. (3)To check whether source of water (tap water or sterile distilled water) makes any difference in the process of disinfection. Methods and Materials: Water samples were collected from dental units of departments of College of Dental Sciences, MDV and 15 Private dental clinics at base line and after disinfection either with Sterilex Ultra or 0.2% CHX for a period of 21 days. Two types of microbial analysis were performed: (i) Coliform count (ii) Colony Forming Unit (CFU) count Statistical analysis: Mean, standard deviation and range was calculated and comparison was done with ADA standard. Results: At base line CFU/ml was higher than the ADA standard. After disinfection either with Sterilex Ultra (three consecutive days then weekly) or 0.2% CHX (daily) dental unit water met ADA standard in College of Dental Sciences and MDV. Conclusion: ADA standard for DUWLs can be achieved with use of sterile distilled water and cleaning of DUWLs either with Sterilex Ultra (three consecutive days then weekly) or 0.2% CHX (daily).

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