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ARTICLE
Year : 2010  |  Volume : 8  |  Issue : 16  |  Page : 174-178

In Vitro Effect of Smokeless Tobacco Extracts on the Growth of Streptococcus Mutans in Relation to Nicotine and Sugar Contents


Post Graduate Student, Post Graduate Student, Post Graduate Student, Reader, Professor and Head, Department of Community Dentistry, College of Dental Sciences, Davangere, Karnataka-577 004, India

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In vitro effect of smokeless tobacco extracts on the growth of Streptococcus mutans in relation to nicotine and sugar contents. Objective: To study the effect of smokeless-tobacco extracts on the growth of Streptococcus mutans. Estimation and assessment of association between sugar and nicotine contents on the growth of Streptococcus mutans. Methodology: Popular brands of unprocessed tobacco, zarda, khaini and snuff were considered for the study. Effect of smokeless tobacco extracts on the growth of Streptococcus mutans was assessed using turbidometric method by reading optical density. Sugar and nicotine contents of smokeless tobacco were estimated using spectrophotometer. Results: Smokeless tobacco has stimulatory effect on the growth of Streptococcus mutans. The enhancing effect on growth of Streptococcus mutans is partially attributed to presence of sugar content in the smokeless tobacco. Nicotine showed no effect on growth of Streptococcus mutans. Conclusion: There is significant association between sugar content of smokeless-tobacco and growth of Streptococcus mutans, irrespective of nicotine content.


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