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ARTICLE
Year : 2011  |  Volume : 9  |  Issue : 18  |  Page : 921-922

Comet assay for DNA damage and repair


1 Reader, Dept. of Periodontics, Hitkarini Dental College and Hospital, Jabalpur, M.P, India
2 Professor, Dept. of Public Health Dentistry, Hitkarini Dental College and Hospital, Jabalpur, M.P., India
3 Professor, Dept. of Pedodontics, Hitkarini Dental College and Hospital, Jabalpur, M.P., India

Correspondence Address:
Rizwan M Sanadi
Reader, Dept. of Periodontics, Hitkarini Dental College and Hospital, Jabalpur, M.P
India
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Source of Support: None, Conflict of Interest: None


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The Comet Assay, also called single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for quantifying and analyzing DNA damage in individual cells. This is one of the techniques used in the area of cancer research for the evaluation of genotoxicity and effectiveness of chemoprevention. Ostling & Johansson developed this technique in 1984 . The Comet Assay can be used to detect DNA damage caused by double strand breaks, single strand breaks, alkali labile sites, oxidative base damage, and DNA cross-linking with DNA or protein. The Comet Assay is also used to monitor DNA repair by living cells. It has been reported that smokers have higher level of DNA damage in nasal epithelial cells and buccal cells. Comet assay is an excellent screening test for exposures that may be DNA damaging even in the oral cavity.


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